| Solution Information | help | |
| Enzyme: | Major prion protein | |
| inhibitor: | BDBM66192 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose responses curves for compounds identified as active in a set of previous experiments entitled, "TRFRET-based cell-based primary high throughput screening assay to identify inhibitors of cell surface Prion Protein (PRPC)" (PubChem AID 720596) and "TRFRET-based cell-based high throughput confirmation assay to identify inhibitors of cell surface Prion Protein (PRPC)" (PubChem AID 743200) and were inactive in a set of experiments entitled, "Counterscreen for inhibitors of cell surface Prion Protein (PrPc): Luminescence-based cell-based high throughput assay to identify compounds that are cytotoxic to LD9 cells (PubChem AID 743201). This assay detects PrPc on the surface of living LD9 fibroblast cells using two PRPC-specific antibodies which carry donor and acceptor fluorophores, respectively. In this assay, the antibodies are directed against distinct, non-overlapping PrPc epitopes. Antibody occupancy of both epitopes of a P | |
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